MetaQUAST evaluates and compares metagenome assemblies based on alignments to close references. It is based on QUAST genome quality assessment tool, but addresses features specific for metagenome datasets:
- Huge species diversity – the tool accepts multiple references and makes multi-genome tables and plots, including Krona charts.
- Commonly unknown species content – the tool automatically detects and downloads reference sequences from NCBI.
- Presence of highly relative genomes – the tool detects chimeric contigs and reports "interspecies misassemblies" in addition to the regular assembly errors types.
MetaQUAST can be fed with multiple assemblies, thus is perfect for comparison.
MetaQUAST is distributed within QUAST package since version 2.2.
MetaQUAST specific sections in the Manual: running, output description
Citation:
Alla Mikheenko, Vladislav Saveliev, Alexey Gurevich,
MetaQUAST: evaluation of metagenome assemblies,
Bioinformatics (2016) 32 (7): 1088-1090. doi: 10.1093/bioinformatics/btv697
First published online: November 26, 2015
MetaQUAST: evaluation of metagenome assemblies,
Bioinformatics (2016) 32 (7): 1088-1090. doi: 10.1093/bioinformatics/btv697
First published online: November 26, 2015
Example of comparison of 4 assemblers on the MetaHIT real dataset generated with MetaQUAST v3.2:
Please help us make QUAST better by sending your comments, bug reports, and suggestions
to alexey.gurevich@helmholtz-hips.de or
posting them on our GitHub issue tracker
(please check that an issue similar to yours is not solved or explained already!).